Regulatory effects of Wuzhuyutang (Evodiae prescription) and its consisting herbs on TPH2 promoter.
- Author:
Yugang WANG
1
;
Fan LEI
;
Xiukun WANG
;
Jun HU
;
Honglei ZHAN
;
Dongming XING
;
Lijun DU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Drugs, Chinese Herbal; chemistry; pharmacology; Evodia; chemistry; Humans; Migraine Disorders; drug therapy; enzymology; genetics; PC12 Cells; Promoter Regions, Genetic; drug effects; Rats; Tryptophan Hydroxylase; genetics; metabolism
- From: China Journal of Chinese Materia Medica 2009;34(17):2261-2264
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo screen the active component of Wuzhuyutang (WZYT, Evodiae prescription) and investigate the regulatory effects of the components in WZYT on the TPH2 promoter, and to explore the possible molecular mechanism of WZYT on migraine.
METHODBy transfecting a TPH2 promoter regulating Red Fluorescent Protein expressing plasmid into PC12 cell, the global fluorescence intensities and calculations of fluorescent cells after components treatment were statistically evaluated.
RESULTDifferent regulatory effects of different components in WZYT with different concentrations on TPH2 promoter were observed.
CONCLUSIONTPH2 promoter drove Red Fluorescent Protein expressing cell line can be used as system screening components targeting TPH2 promoter activity. The possible mechanism of WZYT on migraine may due to its stimulating effects on TPH2 promoter, and promote the synthesis and release of 5-HT in cerebral.
