Genetic diversity of different geographical populations of Rhodiola rosea based on AFLP markers.
- Author:
Qiang WANG
1
;
Xiao RUAN
;
Hao JIANG
;
Qingwen MENG
;
Lihe WANG
Author Information
- Publication Type:Journal Article
- MeSH: Amplified Fragment Length Polymorphism Analysis; China; Genetic Variation; Phylogeny; Polymorphism, Genetic; Rhodiola; classification; genetics
- From: China Journal of Chinese Materia Medica 2009;34(18):2279-2284
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo research the genetic diversity of different Rhodiola rosea geographical populations in Tianshan Mountain, China;
METHODThe genetic diversity of eighteen R. rosea geological populations from six niches was estimated using amplified fragment length polymorphism (AFLP) markers. The data of amplified bands were analyzed by the software POPGENE v1.31 (32-bit) and SPSS.
RESULTThe nine primers employed produced a total of 238 discernable and reproducible amplified fragments. There were 228 polymorphic bands. The percentage of polymorphic bands with in different populations was 95.6%. Genetic diversity analysis showed that average number of alleles per loci was Na = 1.4883, effective number of alleles per loci Ne = 1.3907, Neis gene diversity index H = 0.2170, Shannon's information index I = 0.3108, the percentage of polymorphic loci P = 52.71, genetic differentiation among populations Gst = 0.364; UPGMA cluster analysis based on genetic distance data divided eighteen populations into two clusters: Cluster I composed of twelve populations and Cluster II 6 populations which distributed in attitude upper 3 175 m;
CONCLUSIONOur researches suggest that the best niche of R. rosea was at attitude between 3 150-3 250 m; this region is important for the conservation of R. rosea germplasm resource.
