RAPD analysis on genetic relationship among varieties of Rehmannia glutinosa.
- Author:
Zhi-Gang WU
1
;
Min WANG
;
Lu-Qi HUANG
;
Hong-Yan LIU
;
Fei WANG
;
Qing-Jun YUAN
Author Information
- Publication Type:Journal Article
- MeSH: Analysis of Variance; China; DNA, Plant; analysis; genetics; Ecosystem; Genetic Variation; Phylogeny; Plants, Medicinal; genetics; growth & development; Random Amplified Polymorphic DNA Technique; Rehmannia; classification; genetics; growth & development
- From: China Journal of Chinese Materia Medica 2007;32(18):1865-1869
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo define the genetic relationship and other genetic characters of different varieties of Rehmannia glutinosa.
METHODRAPD reactions were conducted on total 54 samples from 16 varieties and 2 populations of R. glutinosa. The data were analyzed by Phylips, Popgene and Arlequin software.
RESULTThe majority rule consensus tree defined the 54 samples to 3 groups. Population genetics analysis and AMOVA showed that the genetic diversity among varieties/populations much greater than that within varieties/populations, but within group II the genetic diversity among varieties was similar to that within varieties.
CONCLUSIONDue to the long-time asexual propagation of R. glutinosa, some genetic differentiation has been accumulated and fixed within the species. It was shown that the genetic distance between wild population and cultivated varieties was greater than the genetic distance among cultivated varieties. The wild resource of the plant should be paid more attention and studies.