Simultaneous determination of 5 active components in Fructus Cnidii by HPLC.
- Author:
De-Xin WENG
1
;
Tian-Ao LI
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Cnidium; chemistry; Coumarins; analysis; Fruit; chemistry; Furocoumarins; analysis; Methoxsalen; analogs & derivatives; analysis; Plants, Medicinal; chemistry; Reproducibility of Results
- From: China Journal of Chinese Materia Medica 2007;32(18):1883-1885
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop an RP-HPLC method for simultaneous determination of 5 constituents in Fructus Cnidii.
METHODAnalysis was performed on an Alltech C18 (4.6 mm x 250 mm, 5 microm) column. The mobile phases were acetonitrile water and acetic acid with gradient elution. The flow rate was 1 mL x min(-1). The monitoring wavelength was 325 nm and 245 nm. The column temperature was 40 degrees C.
RESULTThe linear response ranges were 1-20 microg x mL(-1) (r = 0.999 9) for xanthotoxin, 1-20 microg x mL(-1) (r = 0.999 9) for isopimpinellin, 11-20 microg x mL(-1) (r = 0.999 8) for bergapten, 100-1 200 microg x mL(-1) (r = 0.999 7) for imperatorin, 100-2 000 microg x mL(-1) (r = 0.999 9) for osthole. The average recoveries were all above 95%.
CONCLUSIONThe method is simple, sensitive and accurate with good reproducibility.
