Study on variation of actinoside C in leaves of Actinidia kolomikta with different growth periods by RP-HPLC.
- Author:
Yong-Ri JIN
1
;
Ming-Yu GUI
;
Juan LU
;
Xu-Wen LI
;
Ji-Qing XU
Author Information
- Publication Type:Journal Article
- MeSH: Actinidia; chemistry; growth & development; Chromatography, High Pressure Liquid; methods; Flavones; analysis; chemistry; Glycosides; analysis; chemistry; Molecular Structure; Plant Leaves; chemistry; growth & development; Plants, Medicinal; chemistry; growth & development; Seasons
- From: China Journal of Chinese Materia Medica 2007;32(18):1898-1900
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine actinoside C in the leaves of Actinidia kolomikta with different growth periods.
METHODThe separation was performed at 25 degrees C on ZORBAX Extend C18 column (4.6 mm x 250 mm, 5 microm), using amixture of methanol and water (51:49) as a mobile phase. The flow rate was 1.2 mL x min(-1), and the wavelength for measurement was 267 nm.
RESULTThe results showed that the contents of actinoside C in the leaves of A. kolomikta were variety in different growth periods. Actinoside C could reach its highest content in the middle ten days of June, then the content would decrease in the middle ten days of July slightly, it could reach their lowest content in the middle ten days of August.
CONCLUSIONThe optimal collective date for A. kolomikta are in the middle ten days of June.