Determination of macrozamin in Rhizoma Heterosmilacis Japonicae by RP-HPLC.
- Author:
Lei QIAO
1
;
Xiao-Hui CHEN
;
Kai-Shun BI
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Liliaceae; chemistry; Methylazoxymethanol Acetate; analogs & derivatives; analysis; Plants, Medicinal; chemistry; Reproducibility of Results; Rhizome; chemistry
- From: China Journal of Chinese Materia Medica 2007;32(18):1900-1902
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a RP-HPLC method for the determination of the content of macrozamin in Rhizoma Heterosmilacis Japonicae.
METHODA Century C18 AQ column (4.6 mm x 250 mm, 5 microm) was used with the mobile phase consisted of water (4:96). The flow rate was 1.0 mL x min(-1). The detection wavelength was set at 215 nm, and the column temperature was 35 degrees C.
RESULTThe calibration curve was linear (r = 0.999 8) in the range of 19.12 - 382.4 microg x mL(-1) for macrozamin, the average recovery of the method was 99.5%, with RSD 2.1% (n = 9).
CONCLUSIONThis method can be used for the quality study of Rhizoma Heterosmilacis Japonicae.
