Effect of total flaveos of Gymostemma pentaphyllum on protein expression of Fas/FasL genes and TNF-alpha concentration in cultured neonatal rat cardiomyocytes with hypoxia-reoxygenation.
- Author:
Li LE
1
;
Xiao-Li GAO
;
Bao-Xing DING
;
Bin-Xiang YUAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Animals, Newborn; Apoptosis; drug effects; Cell Hypoxia; Cells, Cultured; Drugs, Chinese Herbal; isolation & purification; pharmacology; Enzyme-Linked Immunosorbent Assay; Fas Ligand Protein; metabolism; Flavones; isolation & purification; pharmacology; Gynostemma; chemistry; Immunohistochemistry; Myocytes, Cardiac; cytology; drug effects; metabolism; Oxygen Consumption; drug effects; physiology; Plants, Medicinal; chemistry; Rats; Rats, Sprague-Dawley; Tumor Necrosis Factor-alpha; metabolism
- From: China Journal of Chinese Materia Medica 2007;32(18):1925-1927
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of total flaveos of Gymostemma pentaphyllum on the protein expression of apoptosis-associated Fas/FasL gene and tumor necrosis factor-alpha (TNF-alpha) concentration in cultured neonatal rat cardiomyocytes with hypoxia-reoxygenation (H/R).
METHODA cultured primary neonatal rat cardiomyocytes model with H/R was erected, experiments were divided into six groups, (1)control group, (2)H/R group, (3)15 mg x L(-1) TFG plus H/R group, (4)45 mg x L(-1) TFG plus H/R group, (5) 105 mg x L(-1) TFG plus H/R group, (6)105 mg x L(-1) TFG group. TNF-aconcentration in cultured cardiomyocytes with H/R, was determined by ELISA method, the protein expression of Fas/FasL genes were estimated by immunohisto-chemistry.
RESULTAfter cardiomyocytes were made with H/R, Compared with control group, the positive expression index (PEI) of Fas/FasL proteins in cardiomyocytes increased significantly, Compared with H/R groups, the PEI of Fas/FasL proteins were lower significantly in groups with different dosages of TFG (P < 0.05). TFG inhibited the secretion of TNF-alpha from myocardial cells and increased the survival rate of myocardial cells.
CONCLUSIONThe protein expression of apoptosis-associated Fas/FasL genes increased during H/R. The TFG can protect myocardium against H/R injury by decreasing the production of TNF-alpha, downregulating the protein expression of Fas/FasL genes, and then inhibiting myocyte apoptosis.
