Analysis on genetic diversity of different Salvia miltiorrhiza geographical populations in China.
- Author:
Bing WANG
1
;
Yong ZHANG
;
Cheng-Bin CHEN
;
Xiu-Lan LI
;
Rui-Yang CHEN
;
Li CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Amplified Fragment Length Polymorphism Analysis; China; Cluster Analysis; DNA Primers; DNA, Plant; genetics; Genetic Variation; Genetics, Population; Phylogeny; Plants, Medicinal; classification; genetics; Salvia miltiorrhiza; classification; genetics
- From: China Journal of Chinese Materia Medica 2007;32(19):1988-1991
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo research on genetic diversity of different Salvia miltiorrhiza geographical populations in China.
METHODThe genetic diversity of 27 S. miltiorrhiza geographical populations from ten provinces in China was estimated using amplified fragment length polymorphism (AFLP) markers. The data of amplified bands were analyzed by the software POPGENE and SPSS.
RESULTThe ten primers employed produced a total of 528 discernable and reproduceable amplified fragments. There were 476 polymorphic brands. The percentage of polymorphic bands with in different populations was 90.15%. Genetic diversity analysis showed that Neis gene diversity (He) was 0.261 2 and Shannon's genetic diversity index (1) was 0.403 3. The coefficient of gene similarity was 0.504 0-0.789 0 between populations. The cluster map including all samples were obtained by UPGMA. In the map, there were seven cluster groups and one individual outside the groups.
CONCLUSIONThe genetic diversity with in different geographical population of S. miltiorrhiza in China is plentiful.
