Extraction of sex hormone from antler velvet with supercritical CO2.
- Author:
Zhi-Hong XU
1
;
Shu-Fen LI
;
Jin-Yu WANG
;
Ran ZHOU
;
Song-Jiang TIAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antlers; chemistry; Carbon Dioxide; Chromatography, Supercritical Fluid; methods; Estradiol; isolation & purification; Ethanol; Gonadal Steroid Hormones; isolation & purification; Materia Medica; chemistry; Progesterone; isolation & purification; Radioimmunoassay
- From: China Journal of Chinese Materia Medica 2007;32(19):2000-2003
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the method of extraction of sex hormone from antler velvet with supercritical CO2.
METHODSupercritical fluid extraction (SFE) was used to extract sex hormone from antler velvet and radioimmunoassay (RIA) was used to analyze the extracts. The chemical compositions in extracts were identified by GC-MS, TLC and HPLC, respectively.
RESULTThe experimental results indicated that the extraction yield was 1.56% when 85% ethanol was used as co-solvent at temperature of 65 degrees C and extraction pressure of 30 MPa. Estradiol and progesterone in the extracts were 3.07, 776.18 ng x g(-1) respectively.
CONCLUSIONIt is feasible to extract hormone from antler velvet with supercritical CO2.
