Quantitative analysis of adenosine and cordycepin in Cordyceps sinensis and its substitutes with LC-MS-MS.
- Author:
Zhao YANG
1
;
Shao-Yun CHI
;
Chun-Hui ZHANG
;
Aiying WU
Author Information
- Publication Type:Journal Article
- MeSH: Adenosine; analysis; standards; Animals; Chromatography, High Pressure Liquid; methods; Cordyceps; chemistry; classification; Deoxyadenosines; analysis; standards; Quality Control; Reference Values; Reproducibility of Results; Spectrometry, Mass, Electrospray Ionization; methods
- From: China Journal of Chinese Materia Medica 2007;32(19):2018-2021
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a LC-MS-MS method for determination of adenosine and cordycepin in Cordyceps sinensis and it's substitutes.
METHODThe sawple was extracted with. 90% methanol. Multi-reactions monitoring (MRM) technique was adopted.
RESULTThe regression equations and coefficients were Y = 89.04X + 506.85 (r = 0.999 7) for adenosine, Y = 99.66X + 1 251.34 (r = 0.998 8) for cordycepin respectively. The linear range was 5.0-1 000.0 microg x L(-1) for adenosine and cordycepin. The limits of detection (LOD) were 0. 44 microg x L(-1) for adenosine and 0.31 microg x L(-1) for cordycepin, respectively. The average recoveries of adenosine and cordycepin were 98.1% and 97.9%, respectively.
CONCLUSIONThe method was highly sensitive, selective and fast, which can be used for the determination of adenosine and cordycepin in C. sinensis and it's substitutes. This method can also be applied for the quality control of the medicinal materials.
