Studies on bioassay-guided anti-inflammatory fraction in bark of Albizia julibrissin combined determination with LC-MS-MS.
- Author:
Shan-Yi QIAO
1
;
Dong-Hong YU
;
Ji-Fen GUO
;
Yi-Min ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Albizzia; chemistry; Animals; Anti-Inflammatory Agents, Non-Steroidal; analysis; isolation & purification; therapeutic use; Biological Assay; methods; Butanols; Chromatography, High Pressure Liquid; methods; Croton Oil; Drugs, Chinese Herbal; analysis; isolation & purification; therapeutic use; Edema; chemically induced; drug therapy; Glycosides; analysis; isolation & purification; therapeutic use; Lignans; analysis; isolation & purification; therapeutic use; Male; Mice; Phytotherapy; Plant Bark; chemistry; Plants, Medicinal; chemistry; Tandem Mass Spectrometry; methods
- From: China Journal of Chinese Materia Medica 2007;32(19):2021-2025
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo search the anti-inflammatory fraction of Albizia julibrissin.
METHODInflammatory model of Kunming mice ear edema induced by croton oil and determination combined with the LC-MS-MS-guided fractionation and isolation were used.
RESULTThe n-butanol fraction (AJ-B) obtained from the ethanolic extract of the Cortex albiziae was the major active fraction. The lignan glycosides fraction (AJ-B-1), which was further isolated from AJ-B, showed significant anti-inflammatory activity and exhibited dose-dependent relationship in the dose of 5 to 20 mg x kg(-1).
CONCLUSIONThe method of bioassay-guided fractionation and isolation combined with the LC-MS-MS determination may be of benefit to the logical studies on the bioactive fractions or constituents of traditional Chinese materia medica.
