Influence of effective part of Zingiber officinal on expression of monocyte chemotactic protein-1 and adhesion molecules.
- Author:
Yun SONG
1
;
Xin-Bing WEI
;
Hua DING
;
Xiu-Min CHENG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Adhesion; drug effects; Cell Line; Cells, Cultured; Chemokine CCL2; biosynthesis; genetics; Drugs, Chinese Herbal; isolation & purification; pharmacology; Endothelial Cells; cytology; drug effects; metabolism; Enzyme-Linked Immunosorbent Assay; Ginger; chemistry; Humans; Hydrogen Peroxide; pharmacology; Intercellular Adhesion Molecule-1; biosynthesis; genetics; Monocytes; cytology; drug effects; metabolism; Plants, Medicinal; chemistry; RNA, Messenger; biosynthesis; genetics; Random Allocation; Rats; Rats, Wistar; Reverse Transcriptase Polymerase Chain Reaction; Vascular Cell Adhesion Molecule-1; biosynthesis; genetics
- From: China Journal of Chinese Materia Medica 2007;32(19):2062-2065
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of effective parts of Zingiber officinal (EPZ) on the adhesion of ECV-304 cells with monocytes cultivated in vitro and on the expression of monocyte chemotactic protein-1 (MCP-1) and adhesion molecules.
METHODThe model of ECV-304 cell oxidative stress injury was established by hydrogen peroxide (H2O2). Then EPZ-contained blood serum was taken as experimental drug. The adherence of monocytes to endothelial cell were measured by method of rose Bengal. The total RNA of cells was extracted. The intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) and MCP-1 mRNA expression in cells were detected by RT-PCR. MCP-1 protein expression were detected by ELISA.
RESULTEPZ could decrease the adhesion of monocytes with ECV-304 cells obviously. Meanwhile it could diminish the expression of ICAM-1, VCAM-1 and MCP-1 in injured ECV-304 cells.
CONCLUSIONEPZ could inhibit H2O2-induced ICAM-1, VCAM-1 and MCP-1 expression in ECV-304 and could inhibit the adherence of monocytes to endothelial cell, which may result in the protect effect in endothelial cells.
