Studies on quality standard of Yifu ointment.
- Author:
You-ming PU
1
;
Yi ZHANG
;
Rui GU
;
Xian-rong LAI
;
Juan DU
;
Ren-chao LI
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; Drug Combinations; Drugs, Chinese Herbal; chemistry; isolation & purification; standards; Liliaceae; chemistry; Ointments; Plants, Medicinal; chemistry; Quality Control; Reproducibility of Results; Rosaceae; chemistry; Saponins; analysis
- From: China Journal of Chinese Materia Medica 2007;32(22):2368-2370
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a method for the determination of Chonglou saponin I and Chonglou saponin II in Yifu ointment.
METHODThe chromatographic separation was performed on Hypersil ODS2 C18 column (4.6 mm x 150 mm, 5 microm) and IBBM-612111 ODS C18 guard column. Acetonitrile-0.02% phosphoric (43:57) as mobile phase. The flow rate was 1 mL min(-1) and column temperature was set at 40 degrees C. The UV detection wavelength was set 203 nm.
RESULTChonglou saponin I showed a good linear relationship at a range of 0.1024-3.2 microg, r =0.9998, the average recovery was 97.4%, and RSD was 1.8% (n = 6); Chonglou saponin II showed a good linear relationship at a range of 0.064-2.0 microg, r = 0.9999, the average recovery was 101.4%, and RSD was 1.0% (n =6).
CONCLUSIONThe method is accurate with the good reproducibility and can be used for the quality control of Yifu Ointment.
