Determination of flavones in Elsholtzia bodinieri by HPLC.
- Author:
Hai-yun CHEN
1
;
Jian FAN
;
Jian-xin CAO
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Flavones; analysis; Lamiaceae; chemistry; Plants, Medicinal; chemistry; Reproducibility of Results
- From: China Journal of Chinese Materia Medica 2007;32(22):2385-2387
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop an HPLC method for the measurement of flavones in Elsholtzia bodinieri.
METHODAnalysis was carried out on Shimadzu LC-2010A HPLC system. The separation was performed on Agilent Extend-RP C18 column (4. 6 mm x 250 mm, 5 microm), with methanol-acetonitrile-0.2% phosphoric acid as mobile phase, and the flow rate was 1.0 mL min(-1) and the detective wavelength was 280 nm.
RESULTA good linear relationship and the mean recovery was found within the range.
CONCLUSIONThis method is rapid, simple and accurate.
