Extraction and content determination of polysaccharides in Viscum coloratum.
- Author:
Jun WANG
1
;
Yi-fan ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, Ion Exchange; methods; Plant Stems; chemistry; Plants, Medicinal; chemistry; Polysaccharides; analysis; chemistry; isolation & purification; Reproducibility of Results; Viscum; chemistry
- From: China Journal of Chinese Materia Medica 2007;32(22):2387-2390
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a method for extraction and content determination of polysaccharide in Viscum coloratum.
METHODPolysaccharide was extracted by hot water, separated by ultrafiltration and ion-exchange chromatography. The content determination was performed at wavelength 490 nm with phenol-sulfuric acid as a chtomo-genic agent.
RESULTThe content of polyaccharide in V. coloratum, CVPS-III, and CVPS-III-C were respectively 4.93% (RSD 1.04%, n = 3), 43.28% (RSD 1.39%, n =3), 69.55% (RSD 1.62%, n = 3), and the average recovery was 96.07% (RSD 2.54%, n = 5).
CONCLUSIONThe method was simple, rapid, and accurate.
