Effects of Salvia miltiorrhiza on TGF-beta1, ROS and PAI-1 induced by angiotensin II in renal mesangial cells.
- Author:
Jun YUAN
1
;
Yan-fang LU
;
Tao-hou CHEN
;
Xiang-fa ZENG
Author Information
- Publication Type:Journal Article
- MeSH: Angiotensin II; pharmacology; Animals; Blotting, Western; Cells, Cultured; Dose-Response Relationship, Drug; Drugs, Chinese Herbal; administration & dosage; isolation & purification; pharmacology; Gene Expression; drug effects; Injections; Mesangial Cells; drug effects; metabolism; Plants, Medicinal; chemistry; Plasminogen Activator Inhibitor 1; biosynthesis; genetics; RNA, Messenger; genetics; metabolism; Rats; Reactive Oxygen Species; metabolism; Reverse Transcriptase Polymerase Chain Reaction; Salvia miltiorrhiza; chemistry; Transforming Growth Factor beta1; blood; secretion
- From: China Journal of Chinese Materia Medica 2007;32(22):2400-2404
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of Salvia miltiorrhiza on glomerulosclerosis induced by Ang II.
METHODRat mesangial cells were exposed to 100 nmol L(-1) Ang II. Meanwhile, we added S. miltiorrhiza injection of different concentrations to Mcs. PAI-1 mRNA and protein, TGF-beta1 in serum free MEM medium, and the level of cellular reactive oxygen species (ROS) were measured.
RESULTS. miltiorrhiza notably attenuated Ang II induced expression of PAI-1 in a concentration-dependent manner. Meanwhile, S. miltiorrhiza suppressed the production of TGF-beta1 and cellular ROS in mesangial cells.
CONCLUSIONS. miltiorrhiza can alleviate glomerular sclerosis. The renoprotective effects of S. miltiorrhiza may be due to its ability to decrease Ang II -induced PAI-1 and TGF-beta1 secretion and cellular ROS level.
