Determination of loniceroside A and C in Lonicerae japonicae by RP-HPLC.
- Author:
Xiao-Guang ZHANG
1
;
Zhi-Min WANG
;
Xiao-Mei SONG
;
Jing-Jing ZHU
;
Hui-Min GAO
;
Li-Mei LIN
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Flowers; chemistry; Lonicera; chemistry; Plants, Medicinal; chemistry; Reproducibility of Results; Saponins; analysis
- From: China Journal of Chinese Materia Medica 2007;32(20):2149-2210
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop an HPLC method for determination of loniceroside A and loniceroside C in Lonicerae japonicae.
METHODThe analysis was carried out on an Alltech C18 column (4.6 mm x 250 mm, 5 microm) evaluated with methanol-acetonitrile-0.1% glacial acetic acid as mobile phase. Flow rate was at 1.0 mL x min(-1) and the detection wavelength was at 210 nm for UV detection.
RESULTThe calibration curves were linear over the range from 0.15 to 2.25 microg (r = 0.999 9) for loniceroside A and 0.11 to 1.65 microg (r = 0.999 1) for loniceroside C. The average recoveries were 99.9% and 98.3%, respectively.
CONCLUSIONThe contents of Loniceroside A&C are diverse in Flos Lonicerae japonicae in different regions.
