Effects of Haikun Shenxi on expression of platelet-derived growth factor-B and mRNA in renal tissue of rats with adriamycin nephropathy.
- Author:
Zong-Jiang ZHAO
1
;
Kai-Feng LIANG
;
Mei-Juan YANG
;
Xin-Xue ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Blood Urea Nitrogen; Creatinine; blood; Doxorubicin; Drugs, Chinese Herbal; chemistry; isolation & purification; pharmacology; Gene Expression Regulation; drug effects; Glomerular Mesangium; drug effects; metabolism; pathology; Glomerulosclerosis, Focal Segmental; chemically induced; genetics; metabolism; Immunohistochemistry; In Situ Hybridization; Kidney; drug effects; metabolism; pathology; Male; Medicine, Chinese Traditional; Phaeophyta; chemistry; Platelet-Derived Growth Factor; biosynthesis; genetics; Polysaccharides; chemistry; isolation & purification; pharmacology; Proto-Oncogene Proteins c-sis; RNA, Messenger; biosynthesis; genetics; Random Allocation; Rats; Rats, Wistar
- From: China Journal of Chinese Materia Medica 2007;32(20):2156-2161
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of Haikun Shenxi on the expression of platelet-derived growth factor-BB (PDGF-BB) and mRNA in renal tissue of rats with adriamycin nephropathy.
METHODRat model was established by unilateral nephrectomy and injecting adriamycin intraperitoneally. The adriamycin-induced nephrotic rats were randomly divided into 6 groups: normal group, sham operation group, model group, lotensin treatment group, Haikun Shenxi low and high dose treatment groups (0.77, 0.08 mg x kg(-1). Ten weeks later, the 24 hour urine protein and blood biochemistry examinations and renal pathologic changes were observed, and the expression of PDGF-BB and mRNA was measured using immunohistochemical method.
RESULTCompared with model group, proteinuria and the levels of serum creatinine (Scr) , urea nitrogen (BUN) were decreased obviously in both Haikun Shenxi low and high dose groups. The expression of PDGF-BB and mRNA was mostly presented in cytoplasm of renal tubular epithelial cells and mesangial area, and it could be reduced significantly after treatment (P < 0. 05).
CONCLUSIONThe level of PDGF-BB and mRNA is high in renal tissue of adriamycin-induced nephrotic rats. This progress could be effectively inhibited by Haikun Shenxi and the mechanism may be that it can control the excessive expression of PDGF-BB and mRNA.
