Experimental studies of effects of Wujiwan extracts in different compatibilities on motility of isolated colon in guinea pig.
- Author:
Ya-Jie WANG
1
;
Yu DONG
;
Xiao-Xin ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Acetylcholine; pharmacology; Animals; Colon; drug effects; physiology; Coptis; chemistry; Dose-Response Relationship, Drug; Drug Combinations; Drugs, Chinese Herbal; administration & dosage; isolation & purification; pharmacology; Epinephrine; pharmacology; Evodia; chemistry; Female; Gastrointestinal Motility; drug effects; Guinea Pigs; In Vitro Techniques; Male; Muscle Contraction; drug effects; Paeonia; chemistry; Plants, Medicinal; chemistry
- From: China Journal of Chinese Materia Medica 2007;32(20):2161-2165
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of Wujiwan extracts in two different compatibility proportions (named the No. 1 and No. 2 respectively) on motility of isolated colon in guinea pig, and compare the therapeutic effects with each other.
METHODTo observe the function of the No. 1 and No. 2 with different concentrations on colon contraction induced by acetylcholine (Ach) and inhibition induced by adrenalin (Ad) respectively, and calculate the EC, with the method of Bliss.
RESULTThe No. 1 and No. 2 both could significantly inhibit the contraction of colon, and in 15 minutes, there was significant time-dependence and concentration-dependence, and EC50 of the No. 1 was lower than that of No. 2. However, but when the colonic was inhibited by Ad, at the time of 9 min and 15 min, the No. 1 at the level of 300 mg x L(-1), and at the time of 15 min, the No. 2 at the level of 30 mg x L(-1), could both excite the colon significantly (P < 0. 05).
CONCLUSIONOur experiments indicate that the No. 1 and No. 2 both can significantly inhibit the colon contraction in guinea pig, and the No. 1 is superior to the No. 2 to some extent. And at the same time, we also can conclude that the No. 1 and No. 2 have exciting effects on inhibitory colon. Meanwhile, the No. 1 and No. 2 each have its own advantages based on the common therapeutic effects.
