Effect of Rehmannia glutinosa water extraction on insulin resistance and gene expression of resistin in type 2 diabetes mellitus rats.
- Author:
Xiu-Fang LV
1
;
Qing-Yu MENG
;
Xin-Min GUO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Blood Glucose; metabolism; Cholesterol; blood; Diabetes Mellitus, Experimental; blood; genetics; pathology; Diabetes Mellitus, Type 2; blood; genetics; pathology; Drugs, Chinese Herbal; isolation & purification; pharmacology; Electrophoresis, Polyacrylamide Gel; Female; Gene Expression Regulation; drug effects; Hypoglycemic Agents; isolation & purification; pharmacology; Insulin; blood; Insulin Resistance; Male; Plants, Medicinal; chemistry; RNA, Messenger; biosynthesis; genetics; metabolism; Random Allocation; Rats; Rats, Wistar; Rehmannia; chemistry; Resistin; genetics; metabolism; Reverse Transcriptase Polymerase Chain Reaction; Triglycerides; blood
- From: China Journal of Chinese Materia Medica 2007;32(20):2182-2184
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of Rehmannia glutinosa water extraction (RGL) on adipose metabolic disorder and gene expression of resistin in type 2 diabetes mellitus rats.
METHODThe wistar rats model of 2-DM were induced by high calorie feeding and small dose injection of STZ. Rats were randomly divided into diabetic model group, diabetic model treated with RGL (2.4 g x kg(-1) x d(-1)), RGL (1.2 g x kg(-1) x d(-1)), RGL (0. 6 g x kg(-1) x d(-1)) and normal control group. The levels of FPG, FINS, TG, HDL, LDL, CH and IR were measured, and the mRNA expression of resistin was determined by RT-PCR, the protein expression measured by SDS-PAGD at the end of 8 weeks.
RESULTThe gene expression of resistin in RGL group were lower than that of diabetic model (P < 0.01). The levels of FPG, FINS, TG, LDL, CH, IR in RGL group were lower than that of diabetic model (P < 0.05), and HDL were higher (P < 0.05). CONCLUDE: RGL can improve insulin resistance in the experimental 2-DM rats, can effectively ameliorate adipose metabolic disturbance and decline IR and FINS by increasing the gene expression of resistin.
