Cloning and bioinformatics analysis of P450 cDNA in Artemisia annua.
- Author:
Jian-Qiang KONG
1
;
Wei WANG
;
Xiao-Dong ZHENG
;
Ping ZHU
;
Ke-Di CHENG
Author Information
- Publication Type:Journal Article
- MeSH: Amino Acid Sequence; Artemisia annua; enzymology; genetics; Base Sequence; Cloning, Molecular; Cytochrome P-450 Enzyme System; classification; genetics; DNA, Complementary; chemistry; genetics; Molecular Sequence Data; Open Reading Frames; genetics; Phylogeny; Plant Proteins; genetics; Plants, Medicinal; enzymology; genetics; Reverse Transcriptase Polymerase Chain Reaction; Sequence Analysis, DNA; Sequence Homology, Amino Acid
- From: China Journal of Chinese Materia Medica 2007;32(21):2227-2231
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVECloning and bioinformatics analysis of P450 cDNA in Artemisia annua.
METHODA P450 cDNA gene was cloned from A. annua by RT-PCR. The bioinformatics analysis of the P450 gene was performed.
RESULTThe complete ORF of this P450 cDNA is 1 464 bp and encodes 488 aa. The sequence was reported to GenBank and coded as DQ667171. Bioinformatics analysis of the P450 cDNA showed it encoded an A-type P450 protein with 54. 992 kDa, it's isoelectric point was 8.83 and the possibility of export to mitochondria was 0.893 2.
CONCLUSIONThe comparable analysis of the P450 with CYP71AV1 revealed that the two proteins probably performed the same function because of the similar character.
