Molecular Epidemiology of Staphylococcus aureus among Patients with Skin and Soft Tissue Infections in Two Chinese Hospitals.

Fei-fei GU; Ye Chen; De-ping Dong; Zhen Song; Xiao-kui Guo; Yu-xing NI; Li-zhong Han

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Molecular Epidemiology of Staphylococcus aureus among Patients with Skin and Soft Tissue Infections in Two Chinese Hospitals.

Author: Fei-Fei GU ¹ ; Ye CHEN ² ; De-Ping DONG ² ; Zhen SONG ³ ; Xiao-Kui GUO ⁴ ; Yu-Xing NI ¹ ; Li-Zhong HAN ¹
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1. Department of Clinical Microbiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
2. Laboratory, Haian Hospital, Nantong University, Nantong, Jiangsu 226600, China.
3. Faculty of Laboratory Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
4. Department of Microbiology and Parasitology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
Publication Type:Journal Article
MeSH: Adolescent; Adult; Aged; Aged, 80 and over; Anti-Bacterial Agents; pharmacology; Child; Child, Preschool; China; Female; Hospitals; Humans; Infant; Linezolid; pharmacology; Male; Methicillin-Resistant Staphylococcus aureus; drug effects; isolation & purification; Microbial Sensitivity Tests; Middle Aged; Molecular Epidemiology; Soft Tissue Infections; microbiology; Staphylococcal Infections; microbiology; Staphylococcal Skin Infections; microbiology; Staphylococcus aureus; drug effects; isolation & purification; Teicoplanin; pharmacology; Trimethoprim, Sulfamethoxazole Drug Combination; pharmacology; Vancomycin; pharmacology; Young Adult
From: Chinese Medical Journal 2016;129(19):2319-2324
CountryChina
Language:English
Abstract:
BACKGROUNDStaphylococcus aureus is one of the predominant causes of skin and soft tissue infections (SSTIs), but limited data were available regarding the characterization of S. aureus from SSTIs patients in Jiangsu Province in China. We aimed to investigate the molecular epidemiology of S. aureus among SSTIs patients in two hospitals of Jiangsu Province.
METHODSSixty-two patients with SSTIs from two Chinese hospitals in Jiangsu Province were enrolled in this study, and 62 S. aureus isolates were collected from February 2014 to January 2015. S. aureus isolates were characterized by antimicrobial susceptibility testing, toxin gene detection, and molecular typing with sequence type, Staphylococcus protein A gene type, accessory gene regulator (agr) group, and Staphylococcal cassette chromosome mec t ype.
RESULTSSixteen (25.8%) methicillin-resistant S. aureus (MRSA) isolates were detected, and there was no isolate found resistant to vancomycin, teicoplanin, sulfamethoxazole-trimethoprim, and linezolid. The sei was the toxin gene most frequently found, and no lukS/F-PV-positive isolates were detected among the SSTIs' patients. Molecular analysis revealed that ST398 (10/62, 16.1%; 2 MRSA and 8 methicillin-susceptible S. aureus) to be the dominant clone, followed by ST5 (8/62, 12.9%) and ST7 (8/62, 12.9%).
CONCLUSIONSThe livestock ST398 was the most common clone among patients with S. aureus SSTIs in Jiangsu Province, China. Surveillance and further studies on the important livestock ST398 clone in human infections are necessarily requested.