Silencing effect of cell-specific RNA interference plasmid pPSMAe/p-shNS-ploy(A) loaded by transgenic vector Tf-PEG-PEI targeting nucleostemin on prostate cancer cells in vitro.
- VernacularTitle:Tf-PEG-PEI载细胞特异性干扰质粒靶向沉默前列腺癌核干因子的体外实验研究
- Author:
Ran-lu LIU
1
;
Wen-yu WANG
;
Zhi-hong ZHANG
;
Yong XU
Author Information
- Publication Type:Journal Article
- MeSH: Antigens, Surface; genetics; metabolism; Cell Cycle; Cell Line, Tumor; Cell Proliferation; GTP-Binding Proteins; genetics; metabolism; Genetic Vectors; Glutamate Carboxypeptidase II; genetics; metabolism; Humans; Male; Nuclear Proteins; genetics; metabolism; Plasmids; Polyethylene Glycols; Polyethyleneimine; analogs & derivatives; Promoter Regions, Genetic; Prostatic Neoplasms; pathology; RNA Interference; RNA, Messenger; metabolism; RNA, Small Interfering; genetics; Transfection; Transferrin; genetics
- From: Chinese Journal of Oncology 2012;34(10):725-729
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the transgenic efficiency of non-viral vector Tf-PEG-PEI and the cell specific silencing effect of plasmid pPSMAe/p-shNS-ploy(A) on prostate cancer cells.
METHODSPolyethyleneimine (PEI) was modified by using polyethylene glycol and transferrin to synthesize the non-viral vector Tf-PEG-PEI. NS-specific plasmids pPSMAe/p-shNS-ploy(A) and Tf-PEG-PEI were used to transfect prostate cancer LNCap and PC-3 cells. The changes of cell morphology, proliferation ability and cell cycle were studied after down-regulating the NS gene level.
RESULTSTf-PEG-PEI was successfully modified. After transfection, the PSMA-expressing LNCaP cells became larger and showed more pseudopodia, having a tendency to differentiate. Their cell proliferation ability was reduced, and the detection of cell cycle showed a decrease of S phase and an increase of G(1) phase after knocking down NS gene. These targets were not changed in non-PSMA-expresing PC-3 cells.
CONCLUSIONSThe non-viral vector Tf-PEG-PEI has a high ability to transfer targeted gene into target cells. The cellular specificity of short-hairpin RNA transcription driven by PSMAe/p is confirmed by silencing NS gene. The use of cell specific promoter may be an effective strategy of gene therapy for prostate cancer.
