Differentiation of rat bone marrow mesenchymal stem cells into adipocytes and blockage of the differentiation.
- VernacularTitle:大鼠骨髓间充质干细胞向脂肪细胞分化及分化阻断
- Author:
Xue-zhong CHEN
1
;
Jie-yu ZHANG
;
Jia-yu ZENG
;
Rong YANG
;
Fang LIU
;
Jing ZHAO
;
Wei-bo ZHANG
;
Yu-xian WANG
;
Ai-hong MAO
;
Wen-ru TANG
;
Shi-qi LIAO
Author Information
- Publication Type:Journal Article
- MeSH: Adipocytes; cytology; Animals; Bone Marrow Cells; cytology; drug effects; Cell Differentiation; drug effects; Cell Transformation, Neoplastic; Cells, Cultured; Dexamethasone; pharmacology; Drug Combinations; Female; Indomethacin; pharmacology; Insulin; pharmacology; Mesenchymal Stromal Cells; cytology; drug effects; Methylcholanthrene; pharmacology; Rats; Rats, Wistar
- From: Chinese Journal of Oncology 2012;34(11):826-830
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the pathogenesis of tumors by blocking the normal differentiation process of stem cells.
METHODSBone marrow mesenchymal stem cells (BMSCs) from rats were isolated, cultured and purified by whole bone marrow adherence method. The rat BMSCs were induced to differentiate into adipocytes with dexamethasone, insulin and indomethacin. Blockage of the differentiation process was induced by 3-methylcholanthrene (3-MC).
RESULTSThe differentiation experiment showed that at 30 days after the induction, oil red O staining-positive cells occurred with increased intracytolasmic lipid droplets, characteristic for adipocytes. The differentiation blockage experiment showed that at 30 days after induction, the deposits of oil red O staining-cytoplasmic lipid droplets was significantly reduced, indicating that the blocked cells were adipocytes, but not fully differentiated. Morphological identification showed that cell contact inhibition disappeared, abnormal cell nuclei, increased number of micronucleus aberration and karyotype abnormalities, indicating that malignant transformation of the stem cells occurred after the differentiation blockage.
CONCLUSIONSThe results of this study show a blockage of the differentiation of that stem cells at the intermediate phase, and a tendency of malignant transformation of the stem cells. The results of our study provide new evidence that cancer stem cells may be originated by suppression of stem cell differentiation.