Effects of Tiam1 on invasion and metastasis of breast carcinoma and its mechanisms.
- Author:
Yuan-qing WU
1
;
Yue-ying XIE
;
Gang PENG
Author Information
- Publication Type:Journal Article
- MeSH: Breast Neoplasms; metabolism; pathology; Cell Line, Tumor; Female; Gene Expression Regulation, Neoplastic; Gene Silencing; Guanine Nucleotide Exchange Factors; genetics; metabolism; Humans; Lymphatic Metastasis; Mitogen-Activated Protein Kinase 1; metabolism; Mitogen-Activated Protein Kinase 3; metabolism; Neoplasm Invasiveness; RNA, Messenger; metabolism; RNA, Small Interfering; genetics; Signal Transduction; T-Lymphoma Invasion and Metastasis-inducing Protein 1; Transcription Factor AP-1; metabolism; Vascular Endothelial Growth Factor A; metabolism
- From: Chinese Journal of Oncology 2012;34(11):831-834
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the significance of Tiam1 in invasion and metastasis of breast carcinoma and its mechanisms.
METHODSImmunohistochemistry was used to detect Tiam1 expression in tumor tissue of 126 breast carcinomas. Tiam1 was silenced by siRNA in breast carcinoma cell line MDA-MB-435, then the expressions of phosphor-ERK 1, ERK 2 and VEGF were detected, and electrophoretic mobility shift assay (EMSA) was used to examine the transcription activiy of AP-1.
RESULTSThere was a significant relationship between Tiam1 expression and lymph node metastasis (P < 0.05). Furthermore, after silencing of Tiam1, the expressions of phosphor-ERK 1, ERK 2 and VEGF were decreased, and the transcription activity of AP-1 was down-regulated in the MDA-MB-435 cells.
CONCLUSIONTiam1 is closely related with invasion and metastasis of breast carcinoma, and the cascade Tiam1 through ERK, AP-1 and VEGF pathways may play an important role in enhancing angiogenesis, therefore, to promote invasion and metastasis of breast carcinoma.
