Effect of intracoronary adenovirus vector encoding hepatocyte growth factor gene on hematopoietic stem cells mobilization in patients with extensive coronary heart disease.
- Author:
Shu-lan ZHANG
1
;
Zhi-jian YANG
;
You-rong ZHANG
;
Jian DAI
;
Bo CHEN
;
En-zhi JIA
;
Tie-bing ZHU
;
Hui WANG
;
Lian-sheng WANG
;
Zu-ze WU
;
Ke-jiang CAO
;
Wen-zhu MA
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviridae; genetics; Aged; Coronary Disease; blood; Female; Genetic Therapy; Genetic Vectors; Hematopoietic Stem Cell Mobilization; methods; Hepatocyte Growth Factor; genetics; therapeutic use; Humans; Male; Middle Aged; Transfection
- From: Chinese Journal of Cardiology 2007;35(6):504-508
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of intracoronary adenovirus vector encoding hepatocyte growth factor gene (Ad(5)-HGF) on hematopoietic stem cells mobilization in patients with extensive coronary heart disease.
METHODSPatients with extensive coronary heart disease were treated with intracoronary infusion of adenovirus vector encoding hepatocyte growth factor (Ad(5)-HGF 5 x 10(9) pfu) gene plus stent implantation (n = 9) or equal physiological saline plus stent implantation (n = 9). Angioplasty and stent implantation was performed according to standard clinical practice by the femoral approach and blood samples were drawn from each patient at baseline before PCI, 6 to 24 hours and 6 days post procedure. The number of CD34(+), CD38(+) and CD117(+) cells in peripheral blood was analyzed by flow cytometer.
RESULTSThe number of circulating CD34(+) cells in Ad(5)-HGF gene treatment group 6 hours after procedure and the number of circulating CD117(+) cells 6 days post procedure were significantly higher in Ad(5)-HGF gene treatment group than those in the control group (0.104 +/- 0.082 vs. 0.022 +/- 0.012, P = 0.021) and (0.058 +/- 0.058 vs. 0.012 +/- 0.009, P = 0.034), respectively.
CONCLUSIONIntracoronary administration of Ad(5)-HGF could mobilize hematopoietic stem cells into peripheral blood and the consequent role of this observation on myocardial regeneration warrants further detailed studies.
