The effect of anthocyanins on cholesterol efflux from mouse peritoneal macrophage-derived foam cells and its possible molecular mechanism.
- Author:
Min XIA
1
;
Qing WANG
;
Meng-jun HOU
;
Hui-lian ZHU
;
Jing MA
;
Zhi-hong TANG
;
Wen-hua LING
Author Information
- Publication Type:Journal Article
- MeSH: ATP Binding Cassette Transporter 1; ATP-Binding Cassette Transporters; metabolism; Animals; Anthocyanins; pharmacology; Cells, Cultured; Cholesterol; metabolism; Foam Cells; drug effects; metabolism; Macrophages, Peritoneal; cytology; Mice
- From: Chinese Journal of Cardiology 2007;35(6):575-579
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the potential role of anthocyanins on modulating cholesterol efflux in mouse peritoneal macrophage-derived foam cells and related molecular mechanisms.
METHODSThe macrophages were isolated from pathogen-free NIH mice and were loaded with 50 microg/ml oxLDL for 24 hours, newly formed foam cells were then treated with anthocyanins (cyanidin-3-glucoside, Cy-3-g; or peonidin-3-glucoside, Pn-3-g) at the concentrations of 1 micromol/L, 10 micromol/L, 100 micromol/L for 0 to 36 hours, respectively. The enzymatic-fluorescent method was used to determine cholesterol content in culture medium. ABCA1 expressions at mRNA and protein level were detected by real-time PCR and confocal microscope.
RESULTSCholesterol efflux of macrophage-derived foam cells increased in a time- and dose-dependent manner post anthocyanins treatment. ABCA1 expressions at mRNA and protein levels were also significantly enhanced after anthocyanins treatment in these cells and these effects could be blocked by co-treatment with DIDS, an inhibitor of the transport activities of ABCA1 and blocker of apoAI-mediated cholesterol efflux.
CONCLUSIONThese data demonstrate that anthocyanins induce cholesterol efflux from mouse peritoneal macrophage-derived foam cells via regulating ABCA1 expression.