Expressions of beta(3)-adrenoceptor and peroxisome proliferator-activated receptor alpha in human failing heart.
- Author:
Ying TIAN
1
;
Shan-jun ZHU
;
Jiang WANG
;
Zhen-kui LI
;
Rong SONG
;
Ling NIE
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Female; Heart Failure; metabolism; Humans; Male; Middle Aged; Myocardium; metabolism; PPAR alpha; metabolism; Receptors, Adrenergic, beta-3; metabolism
- From: Chinese Journal of Cardiology 2007;35(7):607-610
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the changes on the content of substrate, the activity of correlative enzyme and the mRNA and protein expressions of beta(3)-adrenoceptor and peroxisome proliferator-activated receptor alpha (PPARalpha) in human failing heart.
METHODPapillary muscles from 20 patients with heart failure during mitral valves replacement and 6 control subjects died of non-cardiac accidents were obtained and free fat acid (FFA), lactic acid (LD) and the activity of Na(+)K(+)-ATPase and Ca(2+)Mg(2+)-ATPase, protein and mRNA expressions of beta(3)-adrenergic receptor and PPARalpha were measured.
RESULTIn the failing heart, the contents of fat acid, LD and expression of beta(3)-adrenoceptor mRNA and protein were significantly higher while the activity of Na(+)K(+)-ATPase and Ca(2+)Mg(2+)-ATPase, expressions of PPARalpha at mRNA and protein levels were significantly lower than those in control myocardium.
CONCLUSIONMetabolic remodeling (upregulation of beta(3)-adrenoceptor and downregulation of PPARalpha) might contribute to the pathophysiology of heart failure.
