Effects of peroxisome proliferators activated receptors on caveolin-1 expression in foam cells.
- Author:
Yi-deng JIANG
1
;
Jian-tuan XIONG
;
Jun CAO
;
Gui-zhong LI
;
Shu-ren WANG
Author Information
- Publication Type:Journal Article
- MeSH: ATP Binding Cassette Transporter 1; ATP-Binding Cassette Transporters; metabolism; Caveolin 1; metabolism; Cell Line; Cholesterol; genetics; metabolism; Foam Cells; metabolism; Gene Expression; Humans; Malondialdehyde; metabolism; Monocytes; metabolism; PPAR alpha; metabolism; PPAR gamma; metabolism
- From: Chinese Journal of Cardiology 2007;35(7):661-665
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of peroxisome proliferators activated receptors (PPAR) alpha, gamma ligand on ATP-binding cassette transporter A1 (ABCA1) and caveolin-1 expressions and cholesterol, ox-LDL contents in human monocyte derived foam cells.
METHODMalondialdehyde (MDA) was measured by TBARS method, ox-LDL detected by ELISA method, cholesterol measured by fluorescence spectrophotometric method, ABCA1, caveolin-1 mRNA and protein expressions determined by RT-PCR and Western blot, in human monocytes, foam cells [human monocyte-derived macrophage induced by myristate acetate (PMA) further treated with 50 mg/L ox-LDL for 24 h], foam cells plus 10 micromol/L pioglitazone for 48 h, foam cells plus 5 micromol/L clofibrate for 48 h.
RESULTThe intracellular total cholesterol (TC), free cholesterol (FC), cholesteryl ester (CE), ox-LDL and lipid peroxide were significantly increased and the membrane expressions of ABCA1, caveolin-1 were down-regulated in foam cells compared to monocytes (all P < 0.05) and these changes were significantly attenuated by cotreatment with PPARalpha, gamma ligand.
CONCLUSIONThe anti-atherosclerosis effects of PPARalpha, gamma ligand are related to reducing cholesterol contents and up-regulating ABCA1, caveolin-1 expressions in foam cells.
