The role of calcium-sensing receptor on ischemia/reperfusion-induced rat cardiomyocyte apoptosis.
- Author:
Wei-cai ZHANG
1
;
Wei-hua ZHANG
;
Bo WU
;
Ya-jun ZHAO
;
Quan-feng LI
;
Chang-qing XU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; Disease Models, Animal; Female; Male; Mitochondria, Heart; metabolism; Myocardial Ischemia; metabolism; Myocardial Reperfusion Injury; metabolism; Myocytes, Cardiac; cytology; Rats; Rats, Wistar; Receptors, Calcium-Sensing; metabolism
- From: Chinese Journal of Cardiology 2007;35(8):740-744
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the role of calcium-sensing receptor (CaR) on ischemia/reperfusion-induced rat cardiomyocyte apoptosis.
METHODSThe isolated rat hearts were subjected to 40 min ischemia followed by 2h of reperfusion with or without CaR agonist GdCl3 at the beginning of reperfusion. Control hearts (without ischemia) and ischemic hearts (40 min ischemia without reperfusion) served as controls. The protein expressions of CaR, Bcl-2 and cyt C were detected by Western blot. Cardiomyocyte apoptosis was detected by TUNEL. Mitochondrial potential (Deltaphim) was detected by laser confocal microscopy.
RESULTSCompared to controls groups, the expressions of CaR and apoptotic cells were significantly increased, Deltaphim and expressions of mitochondria cyt C and Bcl-2 were significantly reduced in ischemia/reperfusion hearts with or without GdCl3.
CONCLUSIONCaR was involved in the induction of cardiomyocyte apoptosis during ischemia/reperfusion via mitochondrial pathway.
