T-lymphocyte voltage dependent K(+) channel is upregulated in patients with acute coronary syndrome.
- Author:
Li-fen GUO
1
;
Cun-tai ZHANG
;
Jie WU
;
Nian LIU
;
Li-ping SUN
;
Jun LIU
;
Jin MA
Author Information
- Publication Type:Journal Article
- MeSH: Acute Coronary Syndrome; metabolism; Adult; Aged; Female; Humans; Kv1.3 Potassium Channel; metabolism; Male; Middle Aged; Patch-Clamp Techniques; T-Lymphocytes; metabolism
- From: Chinese Journal of Cardiology 2007;35(9):818-821
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine current density of voltage-gated potassium channels and Kv1.3 express in T-lymphocyte derived from patients with acute coronary syndrome (ACS).
METHODSPeripheral blood mononuclear cells were collected from 12 patients with ACS and 10 control donors. Whole-cell patch clamp technique was used to record the outward K(+) currents (IK) and western blots technique was used to detect the express of Kv1.3 protein in lymphocyte.
RESULTS(1) The current density of voltage-gated potassium channel was significantly higher in ACS patients [(269 +/- 94) pA/pF] than in controls [(191 +/- 64) pA/pF, P < 0.01] while membrane capacitance was similar between the two groups. (2) Kv1.3 protein expression was also significantly increased in ACS patients than in controls (P < 0.01).
CONCLUSIONThe lymphocyte voltage-gated potassium channel is upregulated in patients with ACS suggesting a role of Kv activation in the pathophysiology of ACS.
