Effects of sika pilose antler type collagen on ROS1728 cell and its molecular mechanism.
- Author:
Yan-Shuang WANG
1
;
Su LUO
1
;
Da-Fang ZHANG
2
;
Xiao-Bo QU
2
;
Feng LI
1
Author Information
- Publication Type:Journal Article
- Keywords: molecular mechanisms; rat osteosarcoma osteogenesis sample cell line ROS1728; sika pilose antler type I collagen(SPC-I)
- From: China Journal of Chinese Materia Medica 2016;41(18):3412-3418
- CountryChina
- Language:Chinese
- Abstract: In this paper, effect and molecular mechanism of sika pilose antler type I collagen(SPC-I) of ROS1728 cell were explored. For the SPC-I provides the theory basis for the treatment of osteoporosis. The adherent method was used to cultivate rat osteosarcoma osteogenesis sample cell line ROS1728. The effect of SPC-I on ROS1728 cells proliferation was tested by CCK-8 method. Runx2, osernix, ALP, Coll-I, OC osteogenesis related genes expression was tested by RT-PCR, and Runx2 protein expression was tested by Western-bolt. Results showed that 5 g•L ⁻¹ SPC-I could inhibit ROS1728 cell proliferation, and significantly promote the expression of ROS1728 cell specific transcription factor Runx2 and osterix mRNA, Runx2 protein and marker gene ALP, Coll-I, OC mRNA expression(P<0.01). 2.5 g•L ⁻¹ and 10 g•L ⁻¹ SPC-I could significantly inhibit the ROS1728 cell proliferation(P<0.01), and inhibit the expression of related genes. In conclusion, 5 g•L ⁻¹ SPC-I could inhibit ROS1728 cell proliferation, obviously enhance ROS1728 cell function, promote ROS1728 cell differentiation, maturation.
