Effect of Shouwu Shudi Yin on dopaminegic neurons in MPTP induced Parkinson's disease mouse model.

Reginachizi Tunje; Yang-lie YE; Ahmed Sonauddin; Bhugun Hansraj; Sangye Ngawang; Sharma Shivani; Xiong Zhang; Jian-hong Zhu; Rong-pei Liu

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Effect of Shouwu Shudi Yin on dopaminegic neurons in MPTP induced Parkinson's disease mouse model.

Author: Reginachizi TUNJE ¹ ; Yang-Lie YE ² ; Ahmed SONAUDDIN ¹ ; Bhugun HANSRAJ ¹ ; Sangye NGAWANG ¹ ; Sharma SHIVANI ¹ ; Xiong ZHANG ² ; Jian-Hong ZHU ³ ; Rong-Pei LIU ²
Author Information

1. School of International Studies, Wenzhou Medical University, Wenzhou 325000, China.
2. Department of Neurology, the 2nd Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, China.
3. Department of Preventive Medicine, School of Environmental Science and Public Health, Wenzhou Medical University, Wenzhou 325000, China.
Publication Type:Journal Article
Keywords: Parkinson′s disease; Shouwu Shudi Yin; glutathione peroxidase; tyrosine hydroxylase
From: China Journal of Chinese Materia Medica 2016;41(18):3439-3442
CountryChina
Language:Chinese
Abstract: In order to investigate the effect of Shouwu Shudi Yin on dopaminegic neurons in MPTP induced Parkinson's disease mouse model and the possible mechamism, the experimental mice were randomly divided into 4 groups: control, Shouwu Shudi Yin, MPTP and the treatment (MPTP+Shouwu Shudi Yin) groups. The number of tyrosine hydroxylase (TH) positive cells in the substantia nigra was measured by immunohistochemistry, and mRNA expression of TH and glutathione peroxidase (GPX) were detected by PCR. The results showed that the number of TH positive cells and mRNA expression of TH were significantly reduced in MPTP group compared with the control (P<0.05), and pretreated with Shouwu Shudi Yin didn't show protective effect. Compared to MPTP group, the mRNA expression of four subtypes of GPX were increased in various degrees in the treatment group pretreated with Shouwu Shudi Yin, although the difference was not statistically significant. These indicated that the preventive medication of Shouwu Shudi Yin don't have protective effect on the mice with Parkinson' s disease induced by MPTP, but it may enhance the antioxidant capacity through increasing the expression of GPX.