Research of regulating synthesis of luteolin and luteoloside of Lonicera japonica by LjFNS Ⅱ 1.1 and LjFNS Ⅱ 2.1 treated with 5-azaC.
- Author:
Shuang LIU
1
;
Jian YANG
2
;
Liang-Ping ZHA
2
;
Yu-Yang ZHAO
2
;
Yong LIU
1
;
Yuan YUAN
2
;
Lu-Qi HUANG
2
Author Information
- Publication Type:Journal Article
- Keywords: 5-azaC; Lonicera japonica; flavone synthases Ⅱ; luteolin; luteoloside
- From: China Journal of Chinese Materia Medica 2016;41(19):3597-3601
- CountryChina
- Language:Chinese
- Abstract: This study is aimed to explore the mechanism of catalyzing the synthesis of luteolin and luteoloside by LjFNS Ⅱ 1.1 and LjFNS Ⅱ 2.1.The leaves of Lonicera japonica were treated with different concentrations of 5-azaC(20,40,60,80,100 μmol•L-1) for three periods（1,2,3 d）. Firstly, we cloned LjFNS Ⅱ 1.1 and LjFNS Ⅱ 2.1. Secondly, we analyzed the expression levels of LjFNS Ⅱ 1.1 and LjFNS Ⅱ 2.1 by Real-Time PCR and the contents of luteolin and luteoloside determined by UPLC-MS/MS. The results explained the expression levels of LjFNS Ⅱ 1.1 and LjFNS Ⅱ 2.1 consistent with the content variation of luteolin in general, but there was no significant correlation with the contents of luteoloside. And we found the expression levels of LjFNS Ⅱ 1.1 and LjFNS Ⅱ 2.1 were slightly different. The research indicated that the contents of luteolin and luteoloside got higher by improving the expression levels of LjFNS Ⅱ 1.1 and LjFNS Ⅱ 2.1. This will provide technical support and lay a theoretical foundation for regulating the synthesis of luteolin and luteoloside by LjFNS Ⅱ 1.1 and LjFNS Ⅱ 2.1.
