Partly purification of urinastatin by using affinity chromatography
- Author:
Ngoc Thien Pham
- Publication Type:Journal Article
- Keywords:
urinastatin
- MeSH:
Glycoproteins;
Chromatography, Affinity
- From:Journal of Medical Research
1998;8(4):34-39
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
A human urinary trypsin inhibitor (UTI) was purified by 80% amonium sulfat precipitation, affnity chromatography using trypsin cellulofine gel. Specificity inhibitory activity of solution after affinity chromatography was increase from 0.8 to 29.1 and its recovery was 20.8%. The reslt indicated that binding of trypsin to formyl- cellulofine and the affinity chromatography with trypsin cellulofine gel was successful. Capalary electrophoresis of urine, solutions before and after affinity chromatography also supported the above result.