Chemical studieson hydrophilic constituents of toad skin.

Author: Shao-Kun XIN ¹ ; Nan SI ² ; Hong-Jie WANG ² ; Jian YANG ² ; Bo GAO ³ ; Xiao-Lu WEI ² ; Hai-Yu ZHAO ² ; Bao-Lin BIAN ¹
Author Information

1. School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China.
2. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
3. Anhui China Resources Jin Chan Pharmaceutical Co., Ltd., Huaibei 235000, China.
Publication Type:Journal Article
Keywords: hydrophilic constituents; indolealkylamines; toad skin
From: China Journal of Chinese Materia Medica 2016;41(20):3767-3772
CountryChina
Language:Chinese
Abstract: Fifteen compounds were isolated from the toad skin by a combination of various chromatographic methods including macroporous resin, silica gel, ODS and semi-preparative HPLC. Their structures were identified as 4,5-dimethyl-1,3,4,5-tetrahydropyrrolo[4,3,2-de]quinolin-6-ol(1), serotonin(2), N-methyl serotonin(3), O-methyl bufotenine(4), 1,2,3,4-tetrahydro-6-hydroxy-β-carboline(5), O-methylserotonin(6), glycinebetaine(7), caffeine(8), bufotenine(9), shepherdine(10), tryptophan(11), (5-hydroxy-1H-indol-3-yl)acetic acid(12), 5-hydroxy tryptophol(13), 2-methyl-6-hydroxy-1,2,3,4-tetrahydro-β-carboline(14), bufothionine(15). Among them, compound 1 was a new compound,compound 5 was a new natural product. Compounds 4-8 and 10-14 were separated from toad skin for the first time.