Role of pilose antler polypeptides on replicative senescence of rat chondrocyte.
- Author:
Xiao-dong CHEN
1
;
Jian-hua LIN
Author Information
- Publication Type:Journal Article
- MeSH: Aggrecans; genetics; Animals; Antlers; chemistry; Cell Cycle; drug effects; Cells, Cultured; Cellular Senescence; drug effects; Chondrocytes; cytology; drug effects; metabolism; Collagen Type II; genetics; Glycosaminoglycans; analysis; Male; Peptides; pharmacology; RNA, Messenger; analysis; Rats; Rats, Sprague-Dawley
- From: China Journal of Orthopaedics and Traumatology 2008;21(7):515-518
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the function of pilose antler polypeptides (PAP) on replicative senescence of rat chondrocyte subcultivated in vitro by means of PAP intervention and controlled experiment.
METHODSThe 3rd generation chondrocytes were divided into blank group, and PAP groups with three different concentration of PAP which were passaged to the 4th generation. Meanwhile, the 2nd generation of chontrocytes were used as control group. The chondrocytes in different groups were detected with the method of histochemistry for S-A-beta-gal, flow cytometry for cell life cycle and proliferation index, alcian blue test for the content and structure of GAG of ECM, and RT-PCR for type II collagen and Aggrecan. Then PAP's function was observed regarding the appearance and functional status in the process of chondrocyte's senescence.
RESULTSPAP significantly inhibited chondrocyte's express of S-A-beta-gal (P<0.01), promoted chondrocyte's proliferation (P<0.01), reduced cell content on G1 phase, enhanced the content of GAG, type II collagen and Aggrecan of ECM (P<0.01).
CONCLUSIONPAP can significantly resist rat chondrocyte senescence occurred in subcultivation.
