Recombinant E. coli LLO/OVA induces murine BMDCs maturation via TLR4 and NOD1 receptor and promotes specific cytotoxic T cell immunity.
- Author:
Man XU
1
;
Ming-Shen DAI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antigens, Neoplasm; genetics; pharmacology; Bacterial Toxins; genetics; pharmacology; Cancer Vaccines; genetics; pharmacology; Cell Line, Tumor; Cell Proliferation; drug effects; Cell Survival; drug effects; immunology; Coculture Techniques; Cytokines; immunology; secretion; Dendritic Cells; cytology; drug effects; immunology; metabolism; Enzyme-Linked Immunosorbent Assay; Escherichia coli; genetics; metabolism; Female; Flow Cytometry; Heat-Shock Proteins; genetics; pharmacology; Hemolysin Proteins; genetics; pharmacology; Immunity, Innate; drug effects; Mice; Mice, Inbred C57BL; Nod1 Signaling Adaptor Protein; genetics; physiology; Ovalbumin; genetics; pharmacology; Recombinant Fusion Proteins; genetics; pharmacology; Reverse Transcriptase Polymerase Chain Reaction; T-Lymphocytes, Cytotoxic; drug effects; immunology; Toll-Like Receptor 4; genetics; physiology
- From: Biomedical and Environmental Sciences 2010;23(5):350-356
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo explore the immune stimulation effect of recombinant E.coli LLO/OVA on mice bone marrow-derived dendritic cells (BMDCs) and T lymphocytes in vitro.
METHODSAfter BMDCs stimulated by E.coli LLO/OVA, their Toll-like receptor (TLR) and nucleotide-binding oligomerization domain (NOD) receptor signalling pathway were examined by superarray hybridization; and the priming effect of the vaccine activated BMDCs on CD4(+)T and CD8(+)T was determined by [3H]thymidine uptake and ELISA, the tumor cytotoxic effect of activated CD8(+)T cells was determined by cytotoxic assay.
RESULTSAfter BMDCs were activated by E. coli LLO/OVA via TLR4, NOD1 receptor and NF-κB signalling pathway, the expression of their surface molecules including MHC class I, MHC class II, CD40, CD80 and CD86 significantly up-regulated; the secretion of IL-12 and IFN-γ increased also. The mature BMDCs stimulated the allergic CD4(+)T and CD8(+)T cells proliferation and their IL-2 and IFN-γ secretion, and the activated CD8(+)T cells effectively killed B16-OVA melanoma cells and RMA-S/OVA lymphoma cells in vitro.
CONCLUSIONE.coli LLO/OVA is effective in inducing BMDCs maturation via activating TLR4 and NOD1 receptor signalling pathway and promoting specific anti-tumor T cell immunity in vitro.
