Multi-drug resistance and characteristic of integrons in Shigella spp. isolated from China.
- Author:
Jingyuan ZHU
1
;
Guangcai DUAN
;
Haiyan YANG
;
Qingtang FAN
;
Yuanlin XI
Author Information
- Publication Type:Journal Article
- MeSH: Base Sequence; China; DNA Primers; Drug Resistance, Microbial; Drug Resistance, Multiple; Integrons; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Shigella; drug effects; genetics
- From: Biomedical and Environmental Sciences 2011;24(1):56-61
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the characteristic of integrons and the relationship between integrons and antimicrobial resistance in Shigella spp.
METHODSNinety Shigella strains (83 S. flexneri and 7 S. sonnei) were isolated from the stools of patients in China. Susceptibility to 8 antimicrobials was tested for all isolated strains. PCR, RFLP and sequencing analysis of integrons were applied to all of them.
RESULTSHigh prevalence of multi-drug resistance (95.6%) was identified. Of the isolates 79 (87.8%) carried integrase genes of class 1 integron (3.3%), class 2 integron (10.0%) or both (74.4%). No intI3 was detected in the tested isolates. The prevalence of intI2 was significantly higher in isolates with multi-drug resistance to at least 3 antibiotics than that in isolates with resistance to 2 and less antibiotics (P<0.05). Gene cassettes dfrA17-aadA5, dfrA12-orfF-aadA2 of class 1 integron and dfrA1-sat1-aadA1 of class 2 integron were identified.
CONCLUSIONThe class 2 integron may play a role in the emergence of multi-drug resistance in Shigella spp.
