The effect of 2,5-hexanedione on myelin protein zero expression, and its mitigation using Ginkgo biloba extract.

Lei Zhao; Qingjun Liu; Hong Chen; Huawei Duan; Ping Bin; Qing Liu; Yong Niu; Yufei Dai; Yuxin Zheng

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The effect of 2,5-hexanedione on myelin protein zero expression, and its mitigation using Ginkgo biloba extract.

Author: Lei ZHAO ¹ ; QingJun LIU ; Hong CHEN ; HuaWei DUAN ; Ping BIN ; Qing LIU ; Yong NIU ; YuFei DAI ; YuXin ZHENG
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1. National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing, China.
Publication Type:Journal Article
MeSH: Animals; Dose-Response Relationship, Drug; Environmental Pollutants; toxicity; Gene Expression Regulation; drug effects; Hexanones; toxicity; Male; Myelin P0 Protein; genetics; metabolism; Neuroprotective Agents; pharmacology; Plant Extracts; pharmacology; RNA, Messenger; genetics; metabolism; Rats; Rats, Wistar; Sciatic Nerve; drug effects
From: Biomedical and Environmental Sciences 2011;24(4):374-382
CountryChina
Language:English
Abstract:
OBJECTIVETo investigate the role of myelin protein zero (P(0)) in 2,5-hexanedione (2,5-HD)-induced peripheral nerve injury, and the protective effect of Ginkgo biloba extract (Egb761) on 2,5-HD-induced toxic peripheral neuropathy.
METHODSAfter 4 weeks of treatment with 2,5-HD at different doses (50, 100, 200, 400 mg/kg) in rats, changes in the levels of P(0) in rat sciatic nerves was investigated, and the effect of Egb761 on 2,5-HD-induced toxic peripheral neuropathy was studied.
RESULTSThe blood-nerve barrier (BNB) permeability of the sciatic nerve increased, and the expression of P(0) mRNA and P(0) protein decreased in a dose-dependent manner after treatment with 2,5-HD for 4 weeks. Pretreatment with Egb761 protected against BNB interruption, and inhibited P(0) mRNA and protein reduction during 2,5-HD treatment. Pretreatment with Egb761 significantly reduced loss of body weight (P<0.01) and mitigated gait abnormalities (2.85±0.22) induced by 400 mg/kg 2,5-HD (P<0.01). It also reduced the signs of neurotoxicity induced by 2,5-HD.
CONCLUSION2,5-HD inhibited the expression of P(0) in a dose-dependent manner, and this may be an important mechanism by which toxic peripheral neuropathy is induced by 2,5-HD. Egb761 has a protective effect against 2,5-HD-induced peripheral neurotoxicity in rats.