- Author:
YuMei LIU
1
;
WenZhong ZHANG
;
Ling YONG
;
XiaoHong ZHAO
;
XuDong JIA
;
Ning LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Animals, Genetically Modified; Carps; genetics; Follicle Stimulating Hormone; blood; Genitalia, Male; drug effects; Growth Hormone; genetics; metabolism; pharmacology; Luteinizing Hormone; blood; Male; Rats; Testosterone; blood
- From: Biomedical and Environmental Sciences 2011;24(4):445-449
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo evaluate the androgenic and anti-androgenic effects of GH (growth hormone) transgenic carp in male rats.
METHODSHershberger assay was carried out in castrated male SD rats aged 4-5 weeks. Testosterone propionate (TP) (0.4 mg/kg BW) was administrated for a positive control, GH transgenic carp (3.0 g/kg BW)+TP (0.4 mg/kg BW), parental carp (3.0 g/kg BW) + TP (0.4 mg/kg BW), and flutamide (Flu) (3.0 g/kg BW) were used for negative controls, and vehicle was administered orally for a blank control. All groups were administrated for 10 consecutive days. At the end of the test, animals were anesthetized, then weights of accessory sex organ were measured. Serum testosterone (T), luteinizing hormone (LH), and Follicle-Stimulating Hormone (FSH) levels were detected.
RESULTSThe weights ratios of the accessory sex organs and body weights showed no significant differences between the solvent control and the GH transgenic carp-treated groups. Serum concentrations of FSH, LH, and T of the rats treated with GH transgenic carp + TP showed no significant changes, compared with those treated with TP only.
CONCLUSIONGH transgenic carp does not have any androgenic agonist or antagonist properties in vivo screening tests.