In vitro effect of zilongjin on prostate cancer cell line LNCaP.
- Author:
Xue-song LI
1
;
Yun-yan LIANG
;
Dai-shu WANG
;
Xianhe XIE
;
Changjin SHI
;
Jie CHEN
;
Guofeng SUN
;
Liqun ZHOU
;
Zhaoying XUE
;
Yangun NA
;
Yinglu GUO
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents, Phytogenic; pharmacology; Cell Line, Tumor; Cell Proliferation; drug effects; Drugs, Chinese Herbal; pharmacology; Humans; Male; Neoplasms, Hormone-Dependent; metabolism; pathology; Prostate-Specific Antigen; biosynthesis; genetics; Prostatic Neoplasms; metabolism; pathology; Proto-Oncogene Proteins c-bcl-2; biosynthesis; genetics; Receptors, Androgen; biosynthesis; genetics
- From: Chinese Journal of Integrated Traditional and Western Medicine 2004;24(7):621-624
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of Zilongjin (ZLJ) on human androgen-dependent type of prostate cancer cell line LNCaP.
METHODSMTT assay, flow cytometry and fluorescence microscopy were used to observe the effect of ZLJ in anti-proliferation, cell cycle arresting and apoptosis induction. RT-PCR was used to examine the effect of ZLJ on expressions of prostate marker gene (PSA), androgen receptor (AR), apoptosis related genes (bcl-2 and bax), and Western blot assay was used to detect the effect on protein expression of bcl-2 and bax.
RESULTSZLJ could cause apparent inhibition on proliferation, induce G0/G1 phase arresting and apoptosis in time- and dose-dependent manner on LNCaP cells. The concentration for inhibiting cell growth by 50% (IC50) in 72 hrs was 0.79 mg/ml. ZLJ could down-regulate the expression of PSA, AR, bcl-2 genes and lower bcl-2 protein expression, but showed ineffective on bax protein expression.
CONCLUSIONZLJ displays its anti-tumor effects by way of inhibiting the cell proliferation, arresting the G0/G1 phase, inducing apoptosis, down-regulating PSA, AR, bcl-2 gene expression and lowering bcl-2 protein expressions.
