Effect of borneol on the intercellular tight junction and pinocytosis vesicles in vitro blood-brain barrier model.
- Author:
Yan-ming CHEN
1
;
Ning-sheng WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Blood-Brain Barrier; drug effects; physiology; Bornanes; pharmacology; Cell Line; Cell Membrane Permeability; Epithelial Cells; cytology; Kidney; cytology; Male; Models, Neurological; Pinocytosis; drug effects; Rabbits; Tight Junctions; drug effects
- From: Chinese Journal of Integrated Traditional and Western Medicine 2004;24(7):632-634
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the mechanism of borneol in opening the blood-brain barrier (BBB).
METHODSBorneol contained serum was prepared and using Matin-Darby canine kidney epithelium (MDCKE) cell line as the in vitro BBB model to observe the effects of borneol on intercellular tight junction (ICTJ) and pinocytosis vesicles of BBB model.
RESULTSBorneol reduced the ICTJ and caused increase of the number and enlarged the diameter of vesicles. The ICTJ was opened firstly 4 hrs after borneol treatment, then the pinocytosis was affected 24 hrs later. The effects disappeared 24 hrs after removal of the borneol contained serum, indicating that the above-mentioned effects were reversible.
CONCLUSIONBorneol could obviously loosen the ICTJ in BBB, accelerate the transportation of substance through the intercellular passage, it also could increase the number and volume of pinocytosis vesicles in BBB cells, thus to accelerate the transportation of substance by way of cell pinocytosis.
