Antagonistic effect of yishen ruanjian san contained serum against aristolochic acid in antagonizing human renal interstitial fibroblasts.
- Author:
Jing FANG
1
;
Yi-pu CHEN
;
Yan-fang YANG
;
Wei ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Aristolochic Acids; antagonists & inhibitors; toxicity; Cells, Cultured; Drugs, Chinese Herbal; pharmacokinetics; pharmacology; toxicity; Extracellular Matrix; metabolism; Fibroblasts; drug effects; pathology; Kidney; pathology; Male; RNA, Messenger; metabolism; Rats; Rats, Sprague-Dawley; Serum; Tissue Inhibitor of Metalloproteinase-1; analysis; metabolism; Transforming Growth Factor beta; analysis; metabolism; Transforming Growth Factor beta1
- From: Chinese Journal of Integrated Traditional and Western Medicine 2004;24(9):811-815
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study whether yishen ruanjian san contained serum (S-YRS) could intervene the action of aristolochic acid (AA) in antagonizing human renal interstitial fibroblasts (hRIFs) to induce extracellular matrix (ECM) accumulation.
METHODSAA-Na 40 microg/ml, with or without 10% S-YRS, was co-cultured with hRIFs, then the hRIFs mRNA of transforming growth factor-beta1 (TGF-beta1), connective tissue growth factor (CTGF), plasminogen activator inhibitor-1 (PAI-1), tissue inhibitor of metalloproteinase-1 (TIMP-1) and type I collagen (Col I) in the cultured cells were detected by RT-PCR, and their protein expression monitored with ELISA and Western blot respectively.
RESULTSThe mRNA and protein expression of all the above-mentioned factors were significantly up-regulated by AA-Na (P < 0.05). Excepting PAI-1, the enhanced mRNA and protein expression were significantly down-regulated by S-YRS (P < 0.05).
CONCLUSIONS-YRS could down-regulate the hRIF to promote the expression of ECM synthesis factors and inhibit the ECM degradation factors in hRIFs, so as to antagonize the AA stimulated accumulation of ECM such as Col I.
