Effect of jiantai liquid on expression of estrogen and progesterone receptors in uterus of mice with embryo implantation dysfunction induced by mifepristone.
- Author:
Yan-juan LIU
1
;
Guang-ying HUANG
;
Ming-wei YANG
;
Ping GONG
;
Fu'er LU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Drugs, Chinese Herbal; pharmacology; Embryo Implantation; drug effects; Female; Luteolytic Agents; antagonists & inhibitors; pharmacology; Male; Mice; Mifepristone; antagonists & inhibitors; pharmacology; Receptors, Estrogen; biosynthesis; genetics; Receptors, Progesterone; biosynthesis; genetics; Uterus; metabolism
- From: Chinese Journal of Integrated Traditional and Western Medicine 2004;24(9):816-819
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the molecular mechanism of jiantai liquid (JTL) in improving endometrial receptivity of mice with embryo implantation dysfunction (EID).
METHODSMice model of EID induced by mifepristone were intervened with JTL (Twig of Chinese Taxillus, Red Sage root, Chinese Angelica, Milkvetch root, Chuanxiong rhizome), and sacrificed on day 8 of pregnancy. The endometrial estrogen receptor (ER) and progesterone receptor (PR) protein and their gene expressions were assessed by Western blot and semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR).
RESULTSLevels of ER and PR protein and their gene expressions in the JTL treated group were significantly higher than those in the model group respectively (all P < 0.05), and showed insignificant difference from those in the normal control group (all P > 0.05).
CONCLUSIONJTL could promote the development of endometrium and improve the embryo implantation by way of regulating the levels of ER and PR protein and gene expression in mice with EID.
