Study on effect of berbamine on multidrug resistance leukemia K562/Adr cells.
- Author:
Qing-hua DONG
1
;
Shu ZHENG
;
Rong-zhen XU
;
Qinghua LU
;
Liming HE
Author Information
- Publication Type:Journal Article
- MeSH: ATP-Binding Cassette, Sub-Family B, Member 1; biosynthesis; genetics; Alkaloids; pharmacology; Antineoplastic Agents, Phytogenic; pharmacology; Apoptosis; drug effects; Benzylisoquinolines; pharmacology; Caspase 3; Caspases; biosynthesis; genetics; Drug Resistance, Neoplasm; drug effects; genetics; Humans; K562 Cells; RNA, Messenger; biosynthesis; genetics
- From: Chinese Journal of Integrated Traditional and Western Medicine 2004;24(9):820-822
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect and mechanism of berbamine on the apoptosis of multidrug resistant leukemia K562/Adr cells and in reversing the drug resistance.
METHODSIC50 value of K562/Adr cell was determined with MTT method, cell apoptosis rate was analyzed by flow cytometry with Annexin V FITC-PI assay, with the peak and cell cycle detected by PI staining. At the same time, flow cytometry was also used in determining Caspase-3, P-GP protein expression and drug accumulating capacity in cells, and RT-PCR method was used to analyze the gene expression of mdr-1.
RESULTSBerbamine could inhibit human leukemia K562/Adr cell growth in dose-dependent manner, it could also induce cell apoptosis, increase the protein expression of Caspase-3 and the drug excretion capacity of cells, reduce the mRNA and protein expression levels of mdr-1 gene.
CONCLUSIONBerbamine could activate Caspase-3 to induce human leukemia K562/Adr cell apoptosis, and by reducing mdr-1 gene expression to reverse its multidrug resistance.
