Effect of extract from Tripterygium hypoglaucum on proliferation and apoptosis of vascular smooth muscle cells.
- Author:
Zhuo YU
1
;
Lei MENG
;
Yan-hong GUO
;
Dagang WU
;
Wei GAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Aorta; cytology; Apoptosis; drug effects; Cell Division; drug effects; Cells, Cultured; Coronary Restenosis; prevention & control; Drugs, Chinese Herbal; pharmacology; Male; Muscle, Smooth, Vascular; cytology; Rabbits; Tripterygium; chemistry
- From: Chinese Journal of Integrated Traditional and Western Medicine 2004;24(9):827-830
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of THW-4, an extract from Trypterygium hypoglaucum on proliferation and apoptosis of vascular smooth muscle cells (VSMC).
METHODSVSMC derived from rabbit aorta were cultured in vitro and different concentrations of THW-4 were added in experimental groups. Cell proliferation was evaluated by MTT and apoptosis by transmission electron microscopy (TEM), TUNEL assay and Annexin V-FITC/PI double labelled assay.
RESULTSThe inhibitory effects of THW-4 on proliferation of VSMC displayed dose-time dependently, with the IC50 value of 15.6 microg/L at 48 hrs. Incubated with THW-4 (10-100 microg/L) for 56 hrs, VSMC mainly appeared early stage apoptosis and the percentage of apoptosis was found to raise along with the increase of the THW-4 concentration. Typical images of apoptosis could be observed under TEM and TUNEL assay showed increase of DNA segments with karyorrhexis and pyknosis after THW-4 treatment for 72 hrs. Analysis of cell cycle indicated the THW-4 mainly lead to the blockage of VSMC in G2/M stage.
CONCLUSIONTHW-4 could inhibit the proliferation and induce the apoptosis of VSMC in vitro, suggesting that THW-4 is a potential agent for prevention of restenosis following angioplasty.
