Study on sero-pharmacology of fresh Gecko Swinhonis Gunther freeze-dried powder in inducing cell apoptosis of C6 glioma cells in mice.
- Author:
Ping SONG
1
;
Xue-mei WANG
;
Shuang XIE
;
Dingfang PU
;
Hong FU
;
Gengxin LIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antineoplastic Agents; pharmacology; Apoptosis; drug effects; Drugs, Chinese Herbal; pharmacology; Glioma; chemistry; genetics; pathology; Humans; Male; Materia Medica; pharmacology; Mice; Proto-Oncogene Proteins c-bcl-2; biosynthesis; genetics; Random Allocation; Serum; Tumor Cells, Cultured; bcl-2-Associated X Protein
- From: Chinese Journal of Integrated Traditional and Western Medicine 2004;24(10):919-921
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effect of Gecko Swinhonis Gunther freeze-dried powder (GFP) in inducing apoptosis of C6 glioma cells.
METHODSThirty mice were randomly divided into three groups and treated with intraperitoneally injection of cisplatin, orally taken GFP or distilled water respectively. After treatment for 20 days, the serum of mice was collected for treating the C6 glioma cells. The apoptosis state of cells was observed by morphological examination, flow cytometry and TUNEL method, and the cell apoptosis related gene expression of bcl-2 and bax were determined by S-P immunocytochemical assay.
RESULTSThe C6 glioma cell apoptosis induction of GFP contained serum in vitro could be confirmed by morphological examination, flow cytometry analysis and TUNEL method. Comparison between the GFP treated group and the blank control group on intracellular bcl-2 gene expression showed no difference, but bax gene expression was higher in the GFP treated group.
CONCLUSIONGFP contained serum could induce C6 glioma cell apoptosis, its mechanism might be related with the up-regulation of bax gene.
