Study on effect of arsenic trioxide on adhesion and invasion of human hepatocarcinoma cells in vitro.
- Author:
Hai-qing HUA
1
;
Shu-kui QIN
;
Jin-hong WANG
;
Huiying CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antineoplastic Agents; pharmacology; Arsenicals; pharmacology; Carcinoma, Hepatocellular; metabolism; pathology; Cell Adhesion; drug effects; Cell Line, Tumor; Cell Movement; drug effects; Humans; Hyaluronan Receptors; metabolism; Intercellular Adhesion Molecule-1; metabolism; Liver Neoplasms; metabolism; pathology; Matrix Metalloproteinase 2; metabolism; Mice; Mice, Nude; Neoplasm Invasiveness; Oxides; pharmacology
- From: Chinese Journal of Integrated Traditional and Western Medicine 2004;24(10):922-925
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore that the arsenic trioxide injection (ATI) has the effect in antagonizing adhesion and invasion of human hepatocarcinoma cells (HCC), and its relevant mechanism.
METHODSHuman hepatocellular carcinoma cell line SMMC-7721 and the high metastatic nude mice human HCC in situ transplantation model was taken as the objects of study, the effects of ATI on the SMMC-7721 cell movement and migration, its adhesion with fibronectin (FN) and endothelial cell (EC), as well as the CD44 and MMP-2 gene protein expression in transplanted tumor of the model mice were observed by means of cell movement and migration test, cell adhesion test and immunohistochemical method.
RESULTSATI could significantly inhibit SMMC-7721 cell movement and migration on FN, adhesion with FN and EC, also could lower CD44 and MMP-2 in cancer cells.
CONCLUSIONATI has the effects of antagonizing hepatocarcinoma cell adhesion and invasion, the mechanism may be related with the action of ATI in lowering CD44 and MMP-2 expression in cancer cells.
