The influence of the recombinant lentiviral vectors encoding Hyper-IL-6 on the proliferation of human hepatic cell.
- Author:
Wen-jing SUN
1
;
Bo QIN
;
Tao HUANG
;
Ai-long HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Proliferation; Genetic Vectors; Haptoglobins; genetics; metabolism; Hep G2 Cells; Hepatocytes; cytology; metabolism; Humans; Interleukin-6; genetics; metabolism; Lentivirus; genetics; RNA, Messenger; genetics; metabolism; Recombinant Fusion Proteins; genetics; metabolism; Reverse Transcriptase Polymerase Chain Reaction; Time Factors; Transfection
- From: Chinese Journal of Hepatology 2009;17(8):615-619
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of Hyper-IL-6 on the proliferation of L-02 cells.
METHODSThe recombinant lentiviral vectors encoding Hyper-IL-6 (FIV-Hyper-IL-6), IL-6 (FIV-IL-6) and the lentiviral (FIV) were prepared using the FIV-based lentiviral vectors and the packaging system. The titer of FIV-Hyper-IL-6, FIV-IL-6 and FIV was tested with puromycin. L-02 cells were infected with FIV-Hyper-IL-6, FIV-IL-6, FIV, or mock-infected. The growth rate of L02 cells was analyzed with MTT at different time points after infection. The changes of Haptoglobin in L-02 cells were analyzed with RT-PCR.
RESULTSFIV-Hyper-IL-6, FIV-IL-6 and FIV were successfully constructed, and the titer was 107 pfu/ml. 48 hours after infection, the absorbance of the cells infected with FIV-Hyper-IL-6 was 0.6267+/-0.0256, and the absorbances of FIV-IL-6 infected cells, FIV infected cells and mock-infected cells were 0.5563+/-0.0112, 0.5040+/-0.0078 and 0.4790+/-0.0201, respectively. There were significant differences between the FIV-Hyper-IL-6 group and the other groups (F = 41.09, P less than 0.01). The ratio of the absorbance between haptoglobin mRNA and beta-actin was 0.7030+/-0.0106, 0.3355+/-0.0093, 0.1145+/-0.0076 and 0.1143+/-0.0153, respectively, in FIV-Hyper-IL-6 infected cells, FIV-IL-6 infected cells, FIV infected cells and mock-infected cells. There were significant differences between the FIV-Hyper-IL-6 group and the others (q = 57.5007, P less than 0.01).
CONCLUSIONCompared with IL-6, Hyper-IL-6 is more potent to stimulate proliferation and induce the expression of Haptoglobin in L-02 cells.
